The Transformed Ale

A simple modern pale ale recipe designed to explore the impacts of biotransformation.

Beer Style: 

American Non-Hazy Pale Ale

Batch Size: 

5.50 gallons

Boil Time: 

60 minutes

Original Gravity: 







5.40% ABV


5 lbs Domestic 2-Row

4 lbs Maris Otter

2 lbs Munich


Mashing Instructions: 

Single Infusion - 152°F for 60 minutes


0.75 oz Centennial Pellets 10%AA 60 minutes

1.00 oz Centennial Pellets 10%AA 5 minutes

1.00 oz Mosaic Pellets 12%AA 5 minutes

1.00 oz Centennial Pellets 10%AA Dry Hop (Length TBD based on BioTransform)

1.00 oz Mosaic Pellets 12%AA Dry Hop (Length TBD based on BioTransform)


Wyeast 1318 London Ale III
Wyeast 1056 American Ale

Special Instructions / Notes: 

See experiment notes for how to dry hop the beers accordingly. Aiming for 3-4 days exposure to the hop.

Stepp2's picture
Yeast? from the BOOT

For the Bio Transformation recipe I have 2 ?'s.

!) Will we be doing a starter with this recipe? I've used the WYeast smack pack before on small beers around 1.040. This is a 1.054. After reading the WLP001-no starter vs WYeast 1056 comparison I would guess NO. Then again I've seen many well respected brewers use a starter on a beer of this strength.  If we are, I Could use a little help with pitch rate of a smack pack of WYeast . Call me dumb but I cant figure out their online calculator.  

2) Has anybody plugged this into Beersmith yet? and if so can you share. 

BTW- The recipe looks tasty. Thanks for adding MO and Munich to the 2 Row. I really like the flavor they add. For the first time I'll be ditching the carbon filter water and upgrading to using RO water + salt additions on this recipe. I measured 100 ppm on my TDS after filtration. Per 5gal H2O ill be adding 1 tsp CaCl and 1 tsp Gypsum?

As the BOOT I'm looking for any advice to make this brew day better.   


ejpejp77's picture
RO System

Are you getting RO water from somewhere or do you have an RO system?

I'm kind of an RO noob but I have a friend who has been in the water treatment business for decades.

What my friend told me is that you should definately carbon filter prior to RO if your source water (e.g. municipal tap water) has chloromines.  He says chlorine and chloromines will trash your RO membranes.

Just curious.